Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, stimulates to 134% | Paederia scandens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Paederia scandens | |
dithioerythritol | - |
Paederia scandens | |
iodoacetamide | - |
Paederia scandens | |
iodoacetate | - |
Paederia scandens | |
NEM | - |
Paederia scandens | |
PCMB | - |
Paederia scandens | |
Zn2+ | - |
Paederia scandens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00114 | - |
kaempferol | - |
Paederia scandens | |
0.0133 | - |
UDPglucose | - |
Paederia scandens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Paederia scandens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paederia scandens | - |
var. Mairei | - |
Purification (Comment) | Organism |
---|---|
partial | Paederia scandens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Paederia scandens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Paederia scandens |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 15% loss of activity after 2 days | Paederia scandens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + isorhamnetin | 71% of the activity with kaempferol | Paederia scandens | UDP + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
UDPglucose + kaempferol | - |
Paederia scandens | UDP + kaempferol 3-O-glucoside | - |
? | |
UDPglucose + kaempferol 5,7,4'-trimethyl ether | 4% of the activity with kaempferol | Paederia scandens | UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside | - |
? | |
UDPglucose + myricetin | 43% of the activity with kaempferol | Paederia scandens | UDP + myrecetin 3-O-glucoside | - |
? | |
UDPglucose + quercetin | 76% of the activity with kaempferol | Paederia scandens | UDP + quercetin 3-O-glucoside | - |
? | |
UDPglucose + rhamnetin | 70% of the activity with kaempferol | Paederia scandens | UDP + rhamnetin 3-O-glucoside | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris-HCl buffer | Paederia scandens |
8 | - |
imidazole-HCl buffer | Paederia scandens |